Antibacterial activity of the combination of Mentha spicata and Allium sativum extracts on the growth of Mycobacterium tuberculosis clinical isolates

Background: Tuberculosis (TB) is a disease with high mortality rate in the world, including Indonesia. In addition, problem of Mycobacterium tuberculosis resistance to antibiotics getting higher. Mint leaves and garlic have been shown its ability kill M. tuberculosis. However, it has never studied how combined effect growth.Objective: The major objective this study was investigate anti-mycobacterial activity mint leaves-garlic combination growth inhibition clinical culture tuberculosis.Method: Extraction carried out by maceration using ethanol as solvent. cultures were obtained from samples patients diagnosed new (TBB) MDR- (TBB). test agar dilution method on Lowenstein Jensen (LJ) media.Results: extracts, their can inhibit TBB TBM minimal inhibitory concentration (MIC) 3 mg/mL.Conclusion: are effective inhibiting be used an alternative treatment for disease.Keywords: (Mentha spicata), (Allium sativum), tuberculosis, multidrug-resistant (MDR)-Tuberculosis Intisari Latar belakang: Tuberkulosis (TBC) merupakan penyakit dengan tingkat kematian yang tinggi di dunia, termasuk Selain itu, masalah resistensi bakteri terhadap antibiotik semakin tinggi. Daun spicata) dan bawang putih sativum) telah terbukti dapat membunuh Namun belum pernah diteliti bagaimana efek kombinasi keduanya dalam atau menghambat pertumbuhan Tujuan: Mengetahui aktivitas ekstrak daun isolat klinik.Metode: Ekstraksi dilakukan secara maserasi menggunakan pelarut ethanol. Bakteri uji diperoleh dari sampel klinik pasien terdiagnosis tuberkulosis baru multidrug resistant Uji penghambatan metode dilusi padat pada media (LJ).Hasil: Ekstrak serta maupun konsentrasi terkecil mg/mL.Kesimpulan: efektif berpotensi untuk digunakan sebagai alternatif pengobatan tuberkulosis.Kata kunci: (MDR)-TB